Current schedule of defenses
Population Genetic Structure of Unionid Mussels Across Multiple Gulf
Name: Matthew Harrison
Major Advisor: Dr. Noland Martin
Committee Members: Dr. Chris Nice, Dr. Tim Bonner
8:00 AM; Wednesday, November 9, 2022, Supple 257
Abstract: Understanding patterns of genetic differentiation within and across wildlife populations is a key component to parsing the demographic and ecological processes that govern the spatial structure and evolutionary trends of a given species. As molecular and computational techniques continue to advance, these data have become critical tools in delineating population boundaries as well as informing appropriate management decisions. This study utilized a genotype by sequencing (GBS) approach to study the genetic structure of three threatened mussel species of family Unionidae: Pleurobema riddellii, Fusconaia chunii, and Potamilus amphichaenus. Principal component and admixture analyses of these species demonstrated clear intraspecific differentiation across separate drainages as well as indications of isolation within drainages potentially facilitated by geographic barriers such as river impoundments and local environmental and ecological differences. These data present an effective approach to modeling genetic structure across a landscape that allow for more precise inferences on population boundaries and what variables are primarily shaping them.
Matthew is from Flower Mound, Texas and graduated with a B.S. in wildlife biology at Texas State University. Spending his final undergraduate semester studying abroad in Costa Rica, Matthew went on to work at the Dauphin Island Sea Lab before joining Dr. Martin’s lab. Matthew hopes to continue working in the realm of genetics or wildlife after graduating.
TEMPORAL ACTIVITY PARTITIONING IN A CARNIVORE ASSEMBLAGE FROM NEOTROPICAL SITES
Major Advisor: Dr. Ivan Castro-Arellano.
Committee Members: Dr. Joseph Veech, Dr. Clay Green.
Wednesday, November 9th, 9:00 AM, Supple 153, and on Zoom at: https://txstate.zoom.us/j/96662849464?pwd=MTBPVEFCOGJxY2xBSU80emt5YVJTZz09 Passcode: Predators
Abstract: Mammalian carnivores in the Neotropics have a high impact on the ecological structure and function within their ecosystems. It is therefore important to understand how they interact with their environment and utilize main niche axes (i.e., spatial, trophic, and temporal). Minimizing overlap along the temporal niche could result in less agonistic interactions among sympatric species, thus allowing a degree of species coexistence within a community. Using data from a camera- trapping monitoring program at six Neotropical sites, I evaluated how a multi-family assemblage of carnivores utilize the temporal niche along three temporal domains (i.e., full diel, diurnal, and nocturnal) by analyzing activity patterns in a systematic hierarchal fashion. This assemblage consisted of eight carnivore species: jaguar (Panthera onca), puma (Puma concolor), ocelot (Leopardus pardalis), jaguarundi (Puma yagouaroundi), margay (Leopardus wiedii), South American coati (Nasua nasua), crab eating racoon (Procyon cancrivorus), and tayra (Eira barbara). Using a novel analytical approach, I showed that temporal activity patterns were mostly homogeneous among sites, which supported a data pooling strategy that allowed species comparisons using a wider scope. Temporal activity for all species showed relatively even distributions of diurnal activity (55%) and nocturnal activity (56%). Temporal overlap among species pairs were heterogeneous and dependent of domain being analyzed. The South American coati and tayra were the only species pair that showed indistinguishable activity patterns, independent of temporal domain. At the level of pairwise comparisons, a key finding is that most comparisons indicate temporal partitioning among species (69 of 77 analyses) and ensembles (8 of 9 analyses). However, group wide temporal overlap null model analyses showed a different trend as most results (22 of 27 analyses) showed that overlap within and between groups to be indistinct from the random expectation generated by the null model. Overall, this study revealed evident temporal partitioning among species pairs, while groups were revealed to have more random than coincident temporal overlap. Additionally, results showed homogeneous temporal activity patterns among sites. These observations have meaningful implications for future wildlife management decisions at a wider scale regarding carnivore species in the Neotropics.
Bio: Maggie was born in Grand Junction, Colorado. She earned a B.S. in Wildlife Biology from Texas State University in 2019. She has conducted field work on various projects, including the Goudveld Baboon Project in South Africa and the Denali Alpine Wildlife Project in Alaska, which fortified her love for wildlife photography and field work in remote areas. She joined Dr. Castro-Arellano’s lab in 2020 and was a Program Assistant for Dr. Castro Study in America summer program at the Greater Yellowstone Ecosystem in 2022. After graduating she plans to apply her skills in projects focused on mammalian behavior and is considering a potential pursuit of a PhD in this field.
SEASONAL INFLUENCE OF LIGHT AVAILABILITY ON METABOLISM IN AN URBAN, SPRING-FED RIVER
Name: Matthew R. Stehle
Major Advisor: Dr. Weston Nowlin
Committee Members: Dr. Benjamin Schwartz & Dr. Todd Swannack
Wednesday, Nov 2, 2022, 09:00am, FAB 130
Abstract: In aquatic ecosystems, temperature and light are the primary drivers of ecosystem metabolism, which is composed of gross primary production (GPP) and ecosystem respiration (ER). However, light availability and temperature are frequently seasonally synchronous in aquatic systems outside of the tropics, thus it is difficult to assess the independent influence of each parameter on ecosystem metabolism. In this thesis, I examined the effects of seasonal light availability on both GPP and ER, macrophyte community dynamics, and autotrophic metabolism in the San Marcos River, a physicochemically stable spring-fed system with high perennial macrophyte biomass. I estimated GPP, ER, and net ecosystem production (NEP) for spring, summer, and winter seasons in the upper San Marcos River. Using high- resolution drone imagery and in-field measurements, I determined seasonal biovolume (m3) across four reaches of varying recreational intensity. Finally, I used metabolic chambers to estimate seasonal GPP, respiration (R), and net primary production (NPP) of three macrophyte species and plastic plant analogues of increasing morphological complexity to determine how structural complexity influences metabolism of macrophytes and their epiphytes. I found that both GPP and ER in the upper San Marcos River were strongly correlated with seasonal light availability, indicating autotrophic respiration substantial portion of ER. I found that reach-scale macrophyte coverage and biovolume are largely controlled by recreational activity and not seasonal light availability. Finally, macrophyte and epiphyte metabolism were different across seasons and morphotypes, with the most structurally complex macrophytes exhibiting the greatest epiphyte metabolism. Overall, this study indicates that ecosystem metabolism in thermally stable spring-fed rivers is largely driven by light limitation of autotrophic biomass and that human recreational intensity has the ability to influence ecosystem functioning.
Bio: Matthew Stehle is from Schertz, Texas and graduated from Texas State University with a B.S. in Wildlife Biology in 2019. After graduation, he will continue working in the lab with aquatic insects before pursuing a PhD in plant breeding and genetics in the fall of 2023. He enjoys spending time gardening with his husband Patrick and feeding all sorts of vegetables to his dog Ripley von Bismarck Stehle-Towner.
Morphological and molecular evidence of hybridization and asymmetrical gene flow between endemic Mahonia swaseyi and widespread Mahonia trifoliolata
Major Advisor: Dr. Noland Martin
Committee Members: Dr. Chris Nice, Dr. James Ott
Friday November 11, 2022, 8:00am, Supple 153
Abstract: Patterns of hybridization and asymmetric gene flow among divergent taxa provide unique opportunities to investigate the barriers which isolate and maintain distinct species. Genetic and demographic structure of divergent populations can influence the impact of hybridization, and consequences to interspecific gene flow can be neutral, destructive or creative. The endemic Texas Barberry, Mahonia swaseyi, and Agarita, Mahonia trifoliolata, are locally sympatric within Central Texas where apparent phenotypically intermediate hybrids occur. Population genomic and morphological data were integrated to quantify differences among and variation within these two species. We found evidence of early generation hybrids and asymmetries with respect to later-generation hybridization, with directionality towards M. trifoliolata. A heterogeneous introgression pattern across the genome was revealed using a Bayesian Genomic Cline framework, however M. swaseyi alleles appear to have a selective advantage over M. trifoliolata, as they were found to introgress across species boundaries significantly more often. Hybrids were determined to be more similar to M. trifoliolata across most of the morphological traits measured in this study. The phenotypic variation observed between intermediates and M. trifoliolata and M. swaseyi supports the genomic results of asymmetric introgression between M. trifoliolata and M. swaseyi. For rare plant species such as M. swaseyi, hybridization with a widespread congener can constitute a serious risk to the maintenance of species integrity. However, the findings from this study do not support evidence of M. swaseyi being at risk of genetic swamping, and reproductive isolation appears to be maintaining genetic integrity of the two species despite natural hybridization.
Bio: Avery is from Lafayette, LA and completed her undergraduate degree of Wildlife Biology at Texas State University. Avery studied abroad her last summer of her ungraduated degree in Costa Rica where she studied tropical ecology and conservation biology. Upon graduating, she found herself inspired and motivated to continue her education in conservation biology and joined Dr. Noland Martin’s Population and Conservation Biology lab. Avery plans to continue her education in population and conservation biology.
Activity related to lunar brightness and diel activity patterns of Tapiridae and Felidae species across Neotropical sites
Dr. Ivan Castro-Arellano (Biology Department, Texas State University)
Dr. Clay Green (Biology Department, Texas State University)
Dr. Rafael Reyna-Hurtado (ECOSUR, México)
Friday November 11th, 2022 at 9:30am Supple 257
Abstract: Tapirs serve important ecological roles (e.g., as seed dispersers) and have serious conservation and management needs due to anthropogenic influences of their habitats. However, details of their ecology are not well understood as tapirs are difficult to study because of the secretive nature and usually low densities over wide areas. I used circular statistics and a null model approach to analyze a set of camera trap records (N= 8,889) from 16 sites in Central and South America. I examine the diel activity patterns and amount of activity along lunar phases for the Lowlad tapir (Tapirus terrestris), Mountain tapir (Tapirus pinchaque), and Baird’s tapir (Tapirus bairdii), as well as their main potential predators Jaguar (Panthera onca) and Puma (Puma concolor). For all species I used a null model approach to evince highly chronocoincident activity patterns among sites, thus allowing the examination of activity using a wider scope, instead of a site based traditional approach. Most species showed trends for nocturnal activity patterns. Pairwise comparisons of activity patterns between Tapiridae and Felidae species indicated significant temporal partitioning. Baird’s and Lowland tapirs showed a preference for activity during relatively bright nights (waxing gibbous) whereas Jaguar and Puma showed preference for the brightest nights in the lunar cycle (full moon in both cases). In conclusion, the high degree of temporal partitioning between Tapiridae and Felidae suggests an avoidance of predators regardless of lunar brightness. Results also reveal strong evidence for similar activity patterns across broad geographical scales that allow for more biologically meaningful inferences to be made in relation to wildlife monitoring and conservation in the Neotropics.
Bio: Stefanie is originally from Houston, TX and completed her undergraduate degree from TX State. Once she graduated, she was ready to get her hands dirty working with all the animals she loved so much. Over the years she worked as zoo keeper, emergency care veterinary technician, and also got her wildlife rehabilitation license, as well as spending a couple of summers doing Sea Turtle conservation projects in Costa Rica and Sri Lanka. After much hands-on animal care, she realized she wanted to apply that knowledge and experience to a broader reach in wildlife conservation. Stefanie joined Dr. Ivan Castro’s Wildlife Ecology lab in 2020 and hopes to continue her work in wildlife conservation.
Endangered Fishes, Invasive Parasites, and Migratory Birds: A Habitat Modification Plan Designed to Mitigate Parasite Spillover
Major Advisor: Dr. David G. Huffman – Department of Biology, Texas State University Committee Members:
Dr. Clay Green – Department of Biology, Texas State University
Dr. Ivan Castro-Arellano – Department of Biology, Texas State University
Friday, November 4, 2022 – 1:00pm in FAB 130
The San Marcos and Comal Springs of central Texas contain the only two wild populations of the endangered fountain darter (Etheostoma fonticola). The fountain darter has been negatively impacted by two invasive spillover parasites (Haplorchis pumilio and Centrocestus formosanus) brought in by an invasive thiarid snail, Melanoides tuberculata. These two heterophyid trematodes encyst in the gills, fin-ray insertions, and jaw joints, interfering with functions vital to fish such as respiration, swimming, and feeding. The Green Heron (Butorides virescens) is the principle definitive host for both parasites in central Texas. When an infected bird defecates into the water and an egg is ingested by the snail, the parasite life cycle is completed. This life cycle was studied to determine a vulnerability that could be manipulated by management. Unlike most herons, the Green Heron rarely wades, but forages from banks or emergent structures. Thus, habitat modification is likely the most effective method for parasite mitigation. Structures used by the Green Heron while foraging and roosting were studied from 2021-2022 on Landa Lake, which impounds Comal Springs. A habitat modification plan (HMP) was developed for the phased modification of problematic over-water foraging structures favored by the heron, with phases triggered by the drought stages listed in the Edwards Aquifer Authority Critical Period Management Plan. Floating mats were the most frequented structural category preferred by the Green Heron, accounting for 65% of foraging observations. Thus, the key phase of the HMP is to develop of a strategy for the ongoing removal of floating mats during a Stage 2 (and above) drought, which would not only encourage the Green Heron to forage from banks (and therefore defecate on land), but also improve the aesthetics of the Landa Park area. The remaining structural categories were also ranked in order of use frequency for potential use in more severe droughts. A monitoring phase should follow each modification phase to ensure that the herons are compelled to utilize the banks more often.
Bio: Tania was born and raised in north Texas and from a very early age, she knew that she wanted to pursue a career in the conservation of endangered species. In 2020, she earned her bachelor’s in Wildlife Biology from Texas State University and enjoyed the program so much that she decided to stay and join the Wildlife Ecology graduate program. Upon graduation, she hopes to expand her birding life list and find a job where she can help to develop conservation plans for bird species, her favorite fauna. She eventually plans to earn a PhD in a wildlife- related field so that she can someday serve as a professor and guide future generations of aspiring wildlife biologists.
DO TUFTED TITMICE, BLACK-CRESTED TITMICE, AND THEIR HYBRIDS OCCUPY DIFFERENT TYPES OF HABITAT WITHIN THEIR HYBRID ZONE?
Major Advisor: Dr. Joseph Veech
Committee Members: Dr. James Ott, Dr. Noland Martin
Friday, November 4, 2022, 9:30 AM
Habitat is thought to sometimes play a key role in either facilitating or preventing hybridization between some species, particularly birds. Within a hybrid zone, differences in habitat requirements could spatially separate two species to lessen hybridization. However, hybrid individuals might be best adapted to an intermediate type of habitat also occupied by the parent species such that hybridization is facilitated. Tufted Titmice (Baeolophus bicolor, TUTI) and Black-crested Titmice (Baeolophus atricristatus, BCTI) are sister species that hybridize within a narrow east-west contact zone that extends from central Texas into southwest Oklahoma. The zone corresponds with an ecotone that transitions from closed- canopy deciduous forests in the east that are occupied by TUTI, to arid and open woodlands in the west occupied by BCTI. In central Texas, the zone is impacted by urban and suburban development that creates habitat that could be favorable to hybrids. I hypothesized that landscape-level habitat characteristics found within the TUTI × BCTI hybrid zone would influence the relative abundances of the two parent species and their hybrids. I also expected that hybrids would occupy a habitat type intermediate between that of BCTI and TUTI. Specific objectives were to (1) determine if TUTI, BCTI, and their hybrids associate with different habitat characteristics within the hybrid zone, and (2) determine if there are differences in the habitat associations of titmice belonging to different phenotypic hybrid classifications. For the first objective, I used eBird data and high-resolution GIS land cover data in a grid-based sampling design to analyze the habitat associations of the three titmice types throughout the zone. Logistic regression and AIC model selection revealed that the relative abundances of the three titmice types were most closely associated with percent evergreen forest, mixed forest, and shrub cover. BCTI was associated with higher percentages of evergreen forest, and lower percentages of mixed forest and shrub cover, whereas TUTI had the inverse associations. Further, PCA revealed that hybrids tended to occupy a habitat that was intermediate between that of TUTI and BCTI. Anthropogenic disturbance did not appear to influence relative abundances of BCTI, TUTI, or hybrids. For the second objective, I utilized camera traps and conducted walking surveys at select locations within the hybrid zone to identify titmice of various hybrid phenotypes based on Dixon’s hybrid index applied to plumage characteristics. Habitat associations were analyzed similar to the eBird data but at a finer spatial scale. Locations with only pure BCTI phenotypes had more canopy cover and evergreen forest than locations with hybrid phenotypes. My study demonstrates that within the hybrid zone, BCTI and TUTI retain their different habitat associations that also exist for both species in allopatry. However, habitat is likely facilitating hybridization in that hybrid individuals occupy an intermediate type of habitat that is also used by individuals of the two parent species.
Bio: Carli grew up in San Antonio, Texas. In 2019 she graduated with a B.S. in Environmental Science and a minor in Biology from the University of the Incarnate Word. Afterwards she spent a year working as a part-time aquatic biologist for the San Antonio River Authority, where she developed a passion for wildlife conservation and research. In the fall of 2020, she joined Dr. Joe Veech’s lab to pursue a Masters in Population and Conservation Biology. Although her post- graduation plans are not clear as of yet, she looks forward to utilizing her new found skills in advancing her career.
ASSESSING THE INFLUENCE OF LANDSCAPE CHARACTERISTICS ON BAT FATALITIES AT SOUTH TEXAS WIND ENERGY FACILITIES
Houston L. Kimes
Major Advisor: Dr. Sarah Fritts
Committee Members: Dr. Erin Baerwald - University of Northern British Columbia
Dr. Weston Nowlin - Texas State University
Dr. Sara Weaver – Bowman
Wednesday, November 2, 2022, 12:00 PM
Attend on Zoom:
Although wind energy is a viable renewable energy source, it unintentionally causes bat fatalities by wind turbine blade strikes. Previous research has suggested wind energy facility siting and turbine placement within facilities influence the number of bat fatalities; however, there is a knowledge gap regarding the reasons for the variability. This study occurred in Texas, the leading producer of wind energy and home to the greatest diversity and largest colonies of bats in the United States. My objective was to assess the influence of landscape characteristics surrounding two wind energy facilities in southern Texas and around specific turbines at the facilities on the number of bat fatalities. We systematically searched and collected 1,067 bat carcasses under 200 wind turbines at Hidalgo and Los Vientos Wind Energy Facilities from 2017–2018. Total bat fatality estimates were then calculated by GenEst per species: Tadarida brasiliensis (n = 9663), Lasiurus intermedius (n = 2397), Nycticeius humerali (n = 798), L. ega (n = 740), L. cinereus (n = 331), L. xanthinus (n = 264), L. blossevilli (n = 85), Myotis velifer (n = 26), Nyctinomops macrotis (n = 26), Perimyotis subflavus (n = 5), and unknown spp (n = 1656). I used ArcGIS Pro to spatially analyze landscape characteristics at the two facilities and among the 100 wind turbines at each facility at multiple spatial scales (100 m, 500 m, 1 km, 5 km, and 25 km). Landscape characteristics included landcover types such as barren, crops, grassland, developed, shrub/scrub, hay/pasture, forest, wetlands, and open water, proximity to water sources, elevation, and degree of slope. Using generalized linear models, zero-inflated negative binomial models, and AIC model selection, results indicate that landscape characteristics at the broadest scale examined were most strongly associated with estimated bat fatality rates. I suggest minimization efforts such as acoustic deterrents, pre-construction risk assessments with adequate surveys of the landscape and nearby hibernacula, and a post-construction assessment to reduce bat fatalities. For post construction monitoring, I suggest increased or prioritized fatality monitoring of wind turbines in areas that include the landscape characteristics associated with increased bat fatalities, particularly for curtailment, as it is possible that only individual or a set of wind turbines need to be curtailed instead of all, which maintains the viability and socioeconomic advantages of wind energy.
Bio: Houston Kimes is from a small town south of San Antonio, Texas. She received her Bachelor’s of Science in Wildlife and Fisheries Sciences at Texas A&M University in 2017, following her family tradition as a third-generation Aggie. She spent the 2018 summer conducting various wildlife surveys in Utah which sparked her love for mammals. Houston joined Dr. Sarah Fritts’ lab in 2020 to pursue a Master’s in Wildlife Ecology. This past year Houston accepted a position with Pheasants Forever as a Range and Wildlife Conservationist in Idaho.
A comparison of constitutive and induced immune response in coral colonies of variable symbiont densities.
Name: Isabella Changsut
Major Advisor: Dr. Lauren Fuess
Committee Members: Dr. Robert McLean and Dr. David Rodriguez
Wednesday, 10/26/22, 11:00 AM
RFM 05242, https://txstate.zoom.us/j/92762028989
Abstract: Scleractinian corals form the basis of diverse coral reef ecosystems. However, corals are in swift decline globally, in part due to rising disease prevalence. Most corals are dependent on symbiotic interactions with single-celled algae (family Symbiodiniaceae) to meet their nutritional needs. Preliminary evidence suggests that suppression of host immunity may be essential to this relationship. To explore potential immunological consequences of symbiotic relationships in corals, we investigated constitutive and induced immune activity in the facultatively symbiotic coral, Astrangia poculata. Brown (high symbiont density) and white (low symbiont density) colonies of A. poculata were collected from Rhode Island. First, we compared constitutive immune phenotypes between these two groups. Symbiont density was strongly correlated to several of these immune phenotypes; catalase activity and melanin were significantly positively correlated to symbiont density. Next, we investigated potential variation in induced immune response between the two groups. Colonies of A. poculata with variable symbiont densities were exposed to a pathogenic challenge. We then measured differences in constitutive and induced immunity using transcriptomic approaches. Preliminary results indicate significant differences in both constitutive immunity and response to immune challenge as a result of variable symbiont density. Our results highlight the complex nature of symbiosis-immune interplay in cnidarians and emphasize the need for nuanced approaches when considering symbiosis.
Bio: Issy is from Montclair, New Jersey. She graduated with a B.S. in Marine Biology from Roger Williams University in 2020. Outside of the lab, she enjoys exercising, reading, and spending time outdoors. Upon graduation, she will continue her education here at Texas State as she pursues a PhD in Aquatic Resources and Integrative Biology.
The Natural History, Ecology, and Molecular Detection of JollyVille Plateau Salamanders (Eurycea Tonkawae)
Name: Zachary C. Adcock
Major Advisor: Dr. Michael R.J. Forstner
Committee Members: Drs. James D. Nichols, Benjamin A. Pierce, David Rodriguez, and Benjamin F. Schwartz
Location: Tuesday, 18 October 2022, 8:30am, Norris Room, Supple Science Building
Or join Zoom meeting: Meeting ID 998 2457 1045, Passcode: Adcock https://txstate.zoom.us/j/99824571045?pwd=ajVKK0ZPcjhmTTB1MUZoRWZ1WWVkZz09
The Edwards-Trinity Aquifer system of central Texas provides habitat for approximately 15 species of endemic plethodontid salamanders in the genus Eurycea. These taxa are aquatic and inhabit springs and spring-fed creeks in addition to subterranean water in alluvium and the pores, conduits, and caves of eroded karst limestone. Many of these taxa are protected by the U.S. Fish and Wildlife Service (USFWS) because of their small geographic distributions on a landscape undergoing rapid development. Jollyville Plateau Salamanders (E. tonkawae) occur in the Austin, Cedar Park, and Round Rock metropolitan areas of Travis and Williamson counties, Texas, and this species was listed as federally threatened in 2013 because of concerns for habitat loss, degraded water quality, and reduced water quantity due to urbanization. At the time of listing, the USFWS had to designate critical habitat units (CHUs) and identify important habitat components with only a single peer-reviewed, ecological study published for this species. Therefore, the intent of my dissertation was to inform E. tonkawae conservation, management, and policy in a series of chapters that investigate the species’ reproductive biology, ecology in surface habitat, and detection utilizing molecular techniques. First, I modeled the reproductive phenology of E. tonkawae and provided the first estimates of clutch size. Eurycea tonkawae exhibited seasonal reproduction with oviposition occurring in winter months and an average potential clutch size of less than ten oocytes. Second, I estimated E. tonkawae population parameters in relation to distance from a spring outlet and compared the results to the federal CHU. Eurycea tonkawae relative abundance and relative density decreased with increasing distance from a spring outlet, but individuals also occurred outside of the federal CHUs at some sites. Importantly, the downstream extent of E. tonkawae and the rate of change in relative abundance and relative density were heterogenous among study sites which is incongruent with the uniform federal CHU designation. Third, I estimated E. tonkawae relative abundance among cover object types to assess the USFWS’s description of optimal habitat. Eurycea tonkawae utilized all available cover types, and relative abundance was higher in a cover type the USFWS considers suboptimal habitat (i.e., vegetation) compared to optimal habitat (i.e., rocks). Fourth, I estimated E. tonkawae relative density in relation to multiscale habitat parameters to determine which aspects of habitat influenced salamander density in headwater creeks. Eurycea tonkawae relative density was negatively influenced by the presence of predatory, centrarchid fishes, and this habitat component was more influential than several micro-scale ecological variables previously considered important for this species. Finally, I developed, validated, and applied an eDNA assay using quantitative PCR (qPCR) to provide a survey option for habitats where it is difficult or not practical to visually detect E. tonkawae and other members of the Septentriomolge clade. Estimates of the probability of detection and probability of occurrence were similar between eDNA surveys and concurrent visual encounter surveys demonstrating that eDNA has the potential to be an effective survey technique for these species.
Zach is from Cedar Hill, Texas. He earned a B.S. from the University of Tampa and a M.S. from the University of South Florida. He has worked in the field of endangered and threatened wildlife ecology for the better part of the last 20 years in both Florida and Texas. He is currently a Senior Ecologist at Cambrian Environmental in Austin where he is continuing research on central Texas Eurycea salamanders. His wife, Michelle, is also a wildlife biologist in the Ph.D. program at Texas State University and they have two sons, Davis and Coleman, who are far more wild than any animal they have ever had to study.
Environmental Factors Influencing the Spread and Successful Invasion of Arundo donax in Central Texas
Name: Megan Herod
Major Advisor: Dr. Jason Martina Committee Members: Dr. Jennifer Jensen, Monica McGarrity
Wednesday, August 3, 2022, 10:00 AM
Invasive species are a global concern and are a significant contributor to anthropogenic global change. As invasive species continue to invade new regions, they impact natural ecosystem function and displace native species. The success and impact of invasive species can be tied to species biology and the attributes of the invaded ecosystem. A two-step experimental approach using a greenhouse experiment and remote sensing methodology was used to develop an understanding of the environmental conditions where Arundo donax, a large-statured invasive wetland species, performs best and how it may colonize a new areas. I predicted that Arundo would exhibit higher values for performance-related traits such as aboveground biomass (AGB), belowground biomass (BGB), net photosynthesis, and total tiller length under high light, high soil moisture, and high nutrient conditions because there would be fewer factors limiting growth for this species. I also predicted that the spread of Arundo would be closely tied to major flood events when the transportation of propagules downstream and the scouring of banks leads to colonization opportunities. Eight of the eleven response variables analyzed, including AGB, BGB, total tiller length, and net photosynthesis showed a significant interaction between soil moisture and shade with plants grown in saturated, high light conditions having the highest values for performance related traits. Nutrients were found to influence biomass allocation patterns, with plants grown with added nitrogen and phosphorous exhibiting higher shoot:root and stem:leaf ratios, however nutrients were not found to significantly influence performance related traits. Major flood events were shown to influence the rate of spread of Arundo in the studied stretches of the Guadalupe and Medina rivers in Texas. Understanding how invasive species respond to changes in abiotic factors and what influences their ability to colonize new areas is necessary to make predictions about species expansion and prioritize management efforts and can provide necessary information for the development of ecosystem modeling.
Bio: Megan Herod is from McKinney Texas. She graduated from the University of North Texas with a B.S in Ecology for Environmental Science. In her free time Megan enjoys knitting, cooking, and taking care of her house plants. After graduation Megan will continue employment at Copperhead Environmental Consulting.
The Family Malvaceae (sensu stricto) in Texas
Name: Marquise Gates
Major Advisor: Dr. David E. Lemke
Dr. David Lemke - Department of Biology, Texas State University
Dr. Paula Williamson - Department of Biology, Texas State University Dr. Alan Lievens - Department of Biology, Texas Lutheran University
Thursday, July 14th at 10 am on Zoom: https://txstate.zoom.us/j/93827301629?pwd=VU1ZaW1mcld2M0d4SklvM3QzUmlhQT09
Abstract: Taxonomic keys are helpful devices that allow instructors, students, botanists, and others to identify unknown plant specimens. With respect to the flora of Texas, many of the currently available keys for flowering plant identification have become outdated due to changes in the classification system and may cause confusion f or users when the keys are based on obscure or highly technical characteristics of the plants. A good example is
provided by the mallow family (Malvaceae) in Texas. The mostly widely-available keys to the family are those presented by Correll and Johnston (1970), however, these keys are largely based on technical characteristics that can be difficult for the inexperienced user to recognize or interpret, making accurate identifications difficult and uncertain.
This thesis focuses on producing new keys and a revised taxonomic treatment for the identification of members of the plant family Malvaceae in Texas. The keys are based on first-hand observation of specimens on deposit in the Biology Department Herbarium at Texas State University (SWT), as well as reviews of online specimen data and images and relevant literature citations. Thirty-one genera and 87 species of Malvaceae are recognized in this treatment as being native or naturalized to Texas. A description of each genus is provided, along with keys and descriptions of the Texas species.
Bio: Marquise Gates is from Dallas, Texas. He graduated from Texas Lutheran University with a B.S. in Environmental Biology in 2019. He enjoys drawing digital art, reading light novels, identifying plants in the lab, and watching anime. He plans on pursuing a career working in the field of plant taxonomy and identification.
IBR5 MEDIATED LOCALIZATION OF TIR1 IN PLANT AUXIN RESPONSE
Name: Israel Arellano
Major Advisor: Dr. Nihal Dharmasiri
Dr. Sunethra Dharmasiri – Department of Biology, Texas State University
Dr. Hong-Gu Kang – Department of Biology, Texas State University
Tuesday, July 5, 2022, 11:00 am, Supple 376 (Norris Conference Room)
Abstract: Auxin controls many aspects of plant growth and development, primarily by influencing cell division, cell elongation, and cell differentiation. While the simple model of the ubiquitin mediated 26S-proteosome pathway involving SCFTIR1/AFBs explains auxin induced gene expression, recent studies on INDOLE-3-BUTRYIC ACID RESPONSE5 (IBR5) have shown this model is not without nuance. IBR5 encodes a putative dual specificity phosphatase that plays an important, albeit unknown, role in auxin signaling. In many auxin related mutants such as tir1, the degradation of Aux/IAA proteins is slowed down, but in ibr5 mutant backgrounds, Aux/IAA degradation is enhanced. Furthermore, it has been found that IBR5 exhibits holdase activity and interacts with SGT1b, co-chaperone for the foldase HSP90. Recent work on IBR5 indicates that modulation of IBR5 levels in plant affect the abundance of SCF components required for AUX/IAA degradation. To better characterize the role of IBR5 in plant auxin response, Aux/IAA degradation and SCFTIR1 subcellular localization in various mutant backgrounds were analyzed. Results show that IBR5 stabilizes Aux/IAA proteins by reducing SCFTIR1 and Aux/IAA interactions, thus, modulating auxin response. Furthermore, stabilization of Aux/IAAs is antagonistically regulated by SGT1b. IBR5 also modulates levels of CUL1 and ASK1 of the SCFTIR1/AFBs complex and affects nuclear localization of TIR1.
Bio: Israel Arellano is from Laredo, TX. He graduated from Texas State University with a B.S. in Biology in 2019. He enjoys learning about Roman history, listening to music, and reading books written by people smarter than he. Upon graduation, he will begin a Plant Pathology PhD program at Texas A&M University.
INFLUENCE OF VISUAL SCAFFOLDING ON SCIENCE PRACTICES IN AN OUTDOOR ELEMENTARY SCIENCE INVESTIGATION
Major Advisor: Dr. Kristy Daniel, Department of Biology, Texas State University Committee Members: Dr. Paula Williamson and Dr. Michelle Forsythe
Tuesday, June 21, 2022, 10 am, Zoom
https://txstate.zoom.us/j/92344580662?pwd=T3g5dzZwZ1NpUEJHQUY3eVE2NHB6dz 09 Meeting ID: 923 4458 0662 Passcode: Pollinator
Outdoor education is an incredible tool teachers can use to enhance the learning outcomes of their students, however, formal educators face a host of barriers when attempting to implement it. Among these barriers are pressures to meet the demands of state and national standards and standardized assessments, reduced face-to- face interaction between students and teachers, and lack of experience and training in outdoor education design. Outdoor lessons must incorporate the same high-quality science inquiry as seen in traditional, indoor classrooms, but many teachers do not feel confident when designing or implementing these lessons. This brings light to the importance of investigating design methods that will enhance outdoor investigations and ensure that students are developing NGSS science practices when learning outside.
My thesis investigates visual scaffolding techniques as a tool to facilitate science practices in an outdoor education setting through both researcher and practitioner perspectives. The first manuscript is a qualitative research study that investigated the influence of visual scaffolds on student science practices in an outdoor elementary science activity by implementing two versions of an outdoor science investigation with four local classes of fifth graders. Two classes completed a control design with minimal scaffolding techniques and two classes completed a treatment design integrated with scaffolding techniques. Student responses were coded and analyzed for trends in completion and comprehension regarding science practices such as observation, prediction, data collection, data analysis, and constructing explanations. We found evidence suggesting that visual scaffolds are an effective means of engaging students in and modeling science practices outside. Our findings provide simple design tools to teachers who want to bring the benefits of outdoor education to their students and facilitate meaningful science learning. Additionally, findings from this study contribute simple, cost-effective design techniques to the broader context of informal education. The second manuscript included in this thesis is a practitioner’s piece that expands on the application of the visual scaffolding techniques employed in the first manuscript. We provide examples of how we used each visual scaffolding technique in a fifth-grade outdoor investigation about pollinators, and how these techniques can be utilized during outdoor activities to create more meaningful science inquiry.
Bio: Rachel Seets is from Fort Worth, TX. She graduated from the University of North Texas with a B.A. in Biology. She enjoys identifying plants and birds and can often be found hiking out at Purgatory Creek. Upon graduation, she hopes to pursue a career in environmental education.
An integrated view of how environment shapes physiological stress markers in a widespread toad (Incilius nebulifer)
Major Advisor: Dr. Caitlin Gabor
Dr. Andrea Aspbury, Texas State University,
Dr. Veronika Bókony, "Lendület" Evolutionary Ecology Research Group, Plant Protection Institute (NÖVI)
Dr. Susannah French, Utah State University
Dr. Joseph Veech, Texas State University
June 14, 2022, 10:00AM, IGRM 3203
Abstract: Latitude and urbanization gradients are conglomerates of biotic and abiotic variables that result in interesting trends in vertebrate body size, limb length, and life history. While these trends have been the focus of many studies, the underlying hormonal mechanisms have received much less attention. Glucocorticoids are steroid hormones important to growth and somatic maintenance as well as responding to unpredictable stressors. Studies of geographic variation in glucocorticoid stress response and correlations between baseline glucocorticoid levels and lipid storage, hop performance, and defensive toxin production can help us understand how species might adapt to a changing climate and a changing landscape. I propose to study potential physiological tradeoffs in a widespread toad in Texas (Gulf Coast Toad; Incilius nebulifer) and how those tradeoffs might be mediated by glucocorticoids across a latitudinal and urbanization gradient.
Bio: Dillon was born and raised in Monroe, Utah. He received his B.S. in Biology from Southern Utah University in 2013. After working a few years with various state and federal agencies he decided to continue his formal education at California State University, Northridge. He graduated with his M.Sc. in Biology in 2019. He is Married to Laura Monroe, and they have two kids.
Quantification of endocrine disrupting compounds in central Texas rivers and associations with genomic variation in red shiners
Major Advisor: Dr. Chris C. Nice Committee Members:
Dr. Caitlin R. Gabor - Department of Biology, Texas State University Dr. David Rodriguez - Department of Biology, Texas State University Dr. James R. Ott - Department of Biology, Texas State University
Dr. Timothy H. Bonner - Department of Biology, Texas State University
Monday, May 30, 11 am, in Supple 116 or on Zoom at https://txstate.zoom.us/j/95584367132?pwd=N3p4bzA1cEdMWUcwOE1oQ1dwdFFRZz09 Passcode: 500373
Abstract: Over the past decade, chemical pollution has increased in freshwater systems, including increases in endocrine disrupting compounds. One of the most prevalent of these chemical pollutants is 17α-ethinylestradiol (EE2), a synthetic estrogen used for contraceptives. Exposure to EE2 under laboratory conditions has been demonstrated to alter reproduction, development, and behavior of aquatic organisms. Most water quality assessments, however, have detection thresholds that are substantially higher than most observed concentrations of EE2. Furthermore, detection thresholds are higher than biologically relevant concentrations of EE2 as understood from laboratory studies. In this study, I used a sensitive assay to quantify EE2 concentrations in water and red shiner fish, Cyprinella lutrensis, collected from five central Texas rivers. I detected EE2 in all rivers at concentrations that have been shown to cause adverse effects in aquatic organisms. The observed concentrations of EE2 in water and fish suggest that aquatic organisms in central Texas commonly experience non-trivial exposure to EE2. Additionally, given that EE2 can influence reproduction and disrupt development, EE2 exposure could represent a substantial selective pressure for aquatic organisms. I used a Genotype-Environment- Association (GEA) approach to ask whether genomic variation in red shiners was associated with variation in EE2 concentrations. For this, I generated 36,114 single nucleotide polymorphisms (SNPs) for 298 red shiners from 15 localities in five rivers. GEA analysis using Redundancy Analysis included EE2 concentrations in water and fish as predictors of genomic variation as well as other environmental predictors of water quality and patterns of land use in watersheds. Variance partitioning revealed significant proportions of genomic variation explained by my predictors and complex interactions among them. My results indicate that EE2 represents a significant contaminant in central Texas rivers and that there is much more to learn about the impacts on aquatic organisms.
Bio: Alex was born in Austin, Texas and earned his B.S. in Biology from Texas State University in 2020. He joined Dr. Chris Nice's lab immediately after graduation to pursue a Master's in Population and Conservation Biology. After graduation, Alex will begin the Integrative Biology PhD program at the University of California, Berkeley starting Fall 2022.
Dissertation Proposal Defense
Analysis of upland and riparian habitats as stopover sites for migratory songbirds in Central Texas
Name: Dawn Houston
Major Advisor: Dr. Joseph Veech
Committee Members: Dr. Clay Green, Dr. Sarah Fritts, Dr. Sara Morris (Canisius College)
Dr. Susan Pagano (Rochester Institute of Technology)
Thursday, April 28, 2 PM, Norris Conference Room
Attend in person or by ZOOM: https://txstate.zoom.us/j/97256962309?pwd=OEdJL29CemxvcG16OTN2aVdLRlA5Zz09
Abstract: Migration may be the most perilous and energetically expensive period for migratory birds and it has profound effects on survivorship and subsequent breeding success. High-quality stopover sites, with abundant food resources, allow for rapid refueling of energy stores enabling a songbird to continue on the next portion of its journey. Riparian areas are recognized as important stopover habitats, particularly in the Desert Southwest, whereas upland habitats provide adequate stopover sites in other regions of North America, such as the Midwest. Central Texas lies squarely in the Central Flyway, yet no research to date has investigated stopover habitat in this region. I propose to assess the relative quality of riparian and upland habitats as stopover sites by comparing plasma triglyceride concentrations to indicate refueling performance, plasma uric acid to indicate diet quality, and heterophil to lymphocyte ratios to indicate chronic stress of migratory songbirds. I will also compare fine-scale habitat characteristics, such as the proportion of canopy cover, vegetation height, species composition, and Normalized Difference Vegetation Index (NDVI), between habitat types with regard to migratory songbird abundance. Finally, I will evaluate foraging activity and food abundance (arthropod biomass) to identify which habitat type may be more useful as a resource to migrating songbirds. The goal of my research is to acquire knowledge of stopover habitat ecology in Central Texas that could ultimately inform natural resource agencies and conservation stakeholders of priority habitat for migratory bird conservation.
Bio: Dawn Romaine Houston was born on Long Island, New York. She earned her Bachelor of Science in Molecular Biology at Florida Institute of Technology. Her interest in avian ecology led her to fieldwork across the country and internationally, but ultimately brought her to Central Texas to earn a Master of Science in Wildlife Ecology at Texas State University in 2008. Since then, she has worked primarily with the endangered Golden-cheeked Warbler and the delisted Black-capped Vireo. She co-founded, 7eco10 LLC, an environmental consultancy aiming to assist landowners with conservation easement establishment and management. Dawn recently returned to Texas State to study for a Ph.D. degree.
Infection dynamics of Ophidiomyces ophiodiicola, the causative agent of ophidiomycosis, in Texas
Name: Stephen Forrest Harding
Major Advisor: Dr. David Rodriguez
Dr. Camilla Carlos-Shanley, Texas State University Dr. Paul Crump, Texas Parks and Wildlife
Dr. Sarah Fritts, Texas State University
Dr. Michael R.J. Forstner, Texas State University
Tuesday, May 3rd, 2022, 9AM, Supple Science Bldg. Room 116
Abstract: Ophidiomycosis—caused by the fungal pathogen, Ophidiomyces ophiodiicola (Oo)—is an emerging infectious disease (EID) threatening snake populations in North America, Europe, and Asia. In North America, Oo is widespread and has been linked to the decline of two snake populations. In Texas, there have been two confirmed reports of Oo infections; however, considerable knowledge gaps exist for many essential epidemiological factors in the state. For my dissertation research, I address these knowledge gaps by 1) estimating spatiotemporal distributions and host range of Oo in Texas, 2) using historical incidence combined with phylogenetic and population genetic methods to address the origin of Oo in the state, and 3) using field surveys and laboratory experimentation, I assessed the contribution of biotic and abiotic factors to host- pathogen dynamics. I provided an estimate of the contemporary geographic range for Oo in Texas and used multivariate statistics to show seasonal differences in Oo infection prevalence and differences among snakes from different habitat groups. I used historical patterns of Oo occurrence as evidence of the pathogen’s introduction into the state. Using phylogenetic and population genetic analyses, I show strong evidence supporting Oo as a novel pathogen of Texas snakes. I used laboratory infection experimentation to estimate Oo mortality and assess bacterial assemblage differences between infected and non-infected snakes. I detected no differences in bacterial assemblage structure between experimental groups. However, I detected an increased abundance of pathogenic bacterial operational taxonomic units on a host with a moribund infection, suggesting secondary infections synergize with Oo infection to cause mortality. My research narrowed the knowledge gaps for critical epidemiological factors in Texas and identified areas where knowledge gaps still exist, which is vital for future research into the host-pathogen dynamics of Oo in the state.
Bio: Stephen was born and raised in central Texas. He received his B.S. in Aquatic Biology from Texas State University in 2014, and in 2016, he completed his M.S. in the Aquatic Resources program. In 2017, Stephen started his Ph.D. under Dr. David Rodriguez. He is the proud father of Jace and Paige. His passions are fishing, music, and the pursuit of knowledge.
Regional Assessment of Inland Fish Mortalities Associated with Winter and Cold-Shock Stresses
Name: Steven G. Lopez
Major Advisor: Dr. Timothy H. Bonner, Department of Biology, Texas State University Committee Members:
Dr. Andrea S. Aspbury, Department of Biology, Texas State University
Dr. Sarah Fritts, Department of Biology, Texas State University
Wednesday, April 13, 2022, 2:30pm, FAB 102 & Zoom https://txstate.zoom.us/j/93013183544?pwd=eWViaDdaZmF4Z3Brd2F4QlNhdGg0UT09
Meeting ID: 930 1318 3544 Password: freeze
Winter is generally regarded as a stressful period for ectothermic animals (i.e., Winter Stress Syndrome), which can be exacerbated by cold shock stress associated with major arctic freezes. Although loosely defined, major arctic freezes consist of abnormally colder air, and therefore abnormally colder water temperatures, for several days (e.g., 2021’s Winter Storm Uri). During major arctic freezes in the 1980s and in 2021, 35 million Texas marine and estuarine fishes were killed attributed to cold shock stress. Interestingly, few studies report the effects of winter stress or cold shock stress on fishes in inland waters. Purpose of this study was to describe patterns in cold weather fish mortalities attributed to winter stress and cold-shock stress within inland waters of Texas between 1969 and 2021 using records contained within Texas Parks and Wildlife Department’s Pollution Response Inventory and Species Mortality (PRISM) database. Among 53 years, reports of cold weather inland fish mortalities occurred in 66% of the years with greatest percentages of the reports occurring during three major arctic freezes in 1981, 1983, and 2021. Majority of the reports were from urban areas (79%) and from lentic environments (56%). Sixteen taxa and 1,000,000 individuals were reported killed during the 53 years.
Numbers of inland fish mortalities were greater in years with major arctic freezes than in years without major arctic freezes, attributed primarily to mortalities of non-native fishes (e.g., Blue Tilapia Oreochromis aureus, Suckermouth Catfish Hypostomus plecostomus). Numbers of native fish mortalities, primarily clupeids and catostomids, were not different between years with and without major arctic freezes. The 43,000 inland fish mortalities reported during the three major arctic freezes are in stark contrast to the 35 million marine and estuarine fish mortalities. Proposed mechanisms to explain cold shock mortalities in coastal environments (e.g., species within the northern extent of their range, lack of access to deeper water) are similar in inland environments, yet inland environments do not have the same level of mortalities. Consequently, the disparity between mortalities in coastal and inland environments are not readily discernable at this time.
Bio: A first generation college student, Steven graduated from Texas A&M University Galveston with a B.S. in Marine Biology and Marine Fisheries in 2020. Steven is from Houston, TX and enjoys spending time in rivers and streams, catching fish and hiking throughout the state. After graduation, he hopes to continue working with inland fish, and gaining a better understanding of aquatic systems.
Kyle R. Krebs
Major Advisor: Dr. Jessica Dutton
Committee Members: Dr. Timothy H. Bonner, Dr. Weston H. Nowlin, and Dr. Clay Green
Thursday, April 14, 2022, 12:00 pm, Freeman Aquatic Biology 130 and on Zoom.
Meeting ID: 996 5587 1040 Password: ducks
Abstract: Mercury (Hg) is global pollutant that is toxic to wildlife at low concentrations. In waterbirds, exposure to Hg has resulted in altered breeding behavior, reduced hatching success, and nest abandonment. There have only been a few studies which assessed Hg concentrations in waterbirds in Texas, despite Texas being one of the greatest Hg emitters in the U.S. and an overwintering location for migratory waterbirds. In this study, tissues (muscle, liver, breast feather, wing feather) from 16 species of waterbirds that overwinter in Texas, including gadwall, green-winged teal, hooded merganser, lesser scaup, northern shoveler, redhead duck, red-breasted merganser, and sandhill crane were collected by TPWD licensed hunters from nine locations throughout the state and analyzed for total mercury (THg) using a direct mercury analyzer. I investigated THg concentrations among species and assigned foraging guilds (granivore, herbivore, omnivore, piscivore) and feeding strategies (dabbler, diver, dabbler/diver, wader) to determine which tissues and species had the greatest THg concentrations. I also investigated whether muscle and liver THg concentrations were above known threshold levels for adverse biological effects in birds and determined which species had muscle or liver Hg concentrations exceeding federal (EPA; 0.3 µg/g) and state (TDSHS; 0.7 µg/g) advisory levels for human consumption. I also investigated the relationship between δ13C and δ15N and muscle THg concentrations, and lastly determined if wing or breast feather THg concentrations could be used to predict muscle and liver THg concentrations. Inter- and intraspecific differences were observed between species and assigned foraging guilds and feeding strategies. The greatest THg concentrations were measured in either wing feather or liver for most investigated species. Overall, THg concentrations were greatest in piscivorous diving waterbirds such as hooded merganser and red-breasted merganser but were also elevated in northern shoveler (omnivorous dabbler) and lesser scaup (omnivorous diver). Seven of the 16 investigated species had THg concentrations in their muscle and/or liver tissue that put them at risk of experiencing adverse biological effects. These same species also exceeded EPA and TDSHS MeHg advisory levels for human consumption. Wing and breast feather THg concentrations did not successfully predict muscle or liver THg concentrations in gadwall or redhead duck. The results from this study indicate that Hg accumulates the most in species that primarily consume fish but can be above concentrations known to cause deleterious biological effects in non-fish-eating species. However, since all species are migratory, future studies need to investigate the extent to which Hg accumulates in tissues while overwintering in Texas.
Bio: Kyle is a first-generation college graduate from San Antonio, TX and earned his B.S in Environmental Science from the University of Texas, San Antonio in 2015. His passion for environmental justice and scientific awareness comes from youth obsessions with Steve Irwin and Bill Nye and he aims to use his M.S in Aquatic Resources to honor their legacies through environmental conservation.
It’s Not Easy Being Green: The Effects of Agricultural Practices on Stress Physiology and Immune Function in Tadpoles
Name: Amanda Bryant
Major Advisor: Dr. Caitlin Gabor
Committee Members: Dr. Laure Fuess, Texas State University Dr. David Rodriguez, Texas State University
Dr. Jessica Hua, Binghamton University
Dr. Blake Hossack, USGS Northern Rocky Mountain Research Center
Monday, April 11, 2:00pm, in person Supple 257 or join us on Zoom at https://txstate.zoom.us/j/97957942530?pwd=OWczcEYxR09XK3JiQUMvclRmaGJLZz09 Passcode: 939721
Abstract: As the human population continues to grow, agricultural practices will need to change to feed the world, but industrial agriculture is a leading cause of habitat loss and population declines in wildlife. Exposure to agricultural pollutants can cause a variety of issues for wildlife including dysregulation of hormonal responses, decreased food availability, decreases in immune function, and increases parasite prevalence. Organic agriculture has shown some promise in minimizing the negative impacts of agriculture in some taxa. However, consequences of organic agriculture on more sensitive taxa are not well understood. Amphibians are the most imperiled vertebrate group on the planet. Amphibians are highly sensitive to agricultural pollution and have been used as bioindicators of ecosystem quality. In amphibians, the glucocorticoid (GC) response modulates behavioral and physiological responses to environmental stressors. It also heavily interacts with the immune response. All immune cells have GC receptors and the increase in GCs during acute stress can be immune stimulatory. However, long term elevation of GCs can be immunosuppressive due to anti-inflammatory action of GCs. This immunosuppression coupled with pollution presence can lead to increases in parasitic infection in amphibians. My goal is to understand the links between the glucocorticoid stress response, immune defense, and parasitic infections in amphibians living in conventional and organic agricultural habitats.
Bio: Amanda was born and raised in Capac, Michigan where she currently conducts part of her PhD research. She received her B.S. from Michigan State University in 2015. She worked with reptiles and amphibians in Michigan, Nevada, Florida, and Guam before starting her PhD in 2019.
TRACE ELEMENT CONCENTRATIONS AMONG FUNCTIONAL FEEDING GROUPS IN THE ESTUARINE FOOD WEB IN MIDDLE HEMPSTEAD BAY, LONG ISLAND, NEW YORK
Michaela L. Livingston
Major Advisor: Dr. Jessica Dutton
Committee Members: Dr. Weston H. Nowlin & Dr. M. Clay Green
Monday, April 11, 2022, 12:30 pm, FAB 102 & Zoom
Meeting ID: 996 6799 7232 Password: flounder ___________________________________________________________________________________
Estuaries are productive coastal environments that are vulnerable to contamination from human activities. Functional Feeding Groups (FFGs) are a biological classification system that break down taxa based on feeding ecology and could be a useful organization system to understand trace element concentrations within a food web. Middle Hempstead Bay, home to both recreationally fished species and vulnerable species, is an estuarine ecosystem within the South Shore Estuary Reserve on Long Island, New York, made up of densely clustered salt marsh islands and connecting water channels. This study investigated the concentration of six essential (Co, Cu, Fe, Mn, Se, Zn) and four nonessential (As, Cd, Hg, Pb) trace elements in sediment and 27 species from Middle Hempstead Bay, including primary producers, insects, mollusks, crustaceans, fishes, birds, and terrapins. Functional feeding groups (e.g., algae, vascular plants, herbivorous insects, invertivore fishes, and piscivorous birds) were composed of a subselection of investigated species and trace element concentrations were compared within and among them. The tissue distribution of trace elements was also examined for four species: saltmarsh cordgrass (Spartina alterniflora), summer flounder (Paralichthys dentatus), common tern (Sterna hirundo), and black skimmer (Rycops niger). Sediment trace element concentrations were greatest for Fe, followed by Mn, Zn, Pb, Cu, As, Co, Se, Cd, and Hg. Except for Co and Se, essential trace elements were at greater concentration in biota than nonessential elements. Algae had significantly greater concentrations of trace elements known to biodiminish in food webs (e.g., Co, Pb), whereas piscivorous feeding groups had greater concentrations of elements known to biomagnify (e.g., Hg). For most trace elements, the concentration was greatest in the roots for saltmarsh cordgrass and liver in summer flounder. Trace element concentrations in common tern tissues were predominantly greatest in liver compared to muscle and feather. Cadmium, Co, and Hg concentrations were similar across black skimmer tissues, and varied across tissue type for the remaining trace elements. This study is the most comprehensive investigation of trace element concentrations within the Middle Hempstead Bay food web. While FFGs proved useful in assessing trace element accumulation, future research is needed to streamline sampling methodology to reduce biological variability that can overshadow trace element concentration patterns among FFGs.
Bio: Michaela was born and raised in San Antonio, TX and now resides in Lockhart with their husband, Paul, and four beloved hounds (Atlas, Bonnie, Cue, Duncan). Michaela earned a B.S. in Biology and B.F.A. in Studio Art from Texas State University, where they solidified their passion for ecotoxicology, communicating science through art, and facilitating science for others. After graduation, they aim to continue their pursuit of aquatic biomonitoring, in hopes of contributing to the conservation of these key ecosystems for the next generation of folks and wildlife.
Characterization of a Novel Bacterium that Preferentially Grows in Low Fluid Shear Modeled Microgravity
Major Advisor: Dr. Robert JC McLean
Dr. David Rodriguez, Dr. Camila Carlos-Shanley, Dr. Jiseon Yang (Arizona State University)
Tuesday April 12, 2022, at 2pm CDT
Meeting ID: 940 7443 9314 Passcode :186202
Abstract: Multiple spaceflight and microgravity analog studies have increased our understanding of the biological effects of microgravity. Microgravity or modeled microgravity conditions demonstrate changes in microorganisms including virulence, biofilm formation, gene regulation and expression, and other aspects of physiology. These studies were designed to study microgravity functional impacts on individual organisms, but not as a preferential growth condition. Testing for preferential growth in reduced gravity in some organisms may increase our understanding of the gravitational effect on microorganisms. A novel Exiguobacterium species (A1) was isolated from a laminarly-flowing region of the San Marcos River, which has previously been identified as an organism which may display a preference for growth in a low shear modeled microgravity (LSMMG) environment. Members of the genus Exiguobacterium are Gram-positive bacilli that are often extremophiles. Exiguobacterium A1 shows a preference for low fluid shear growth when cultured in the lab but is difficult to propagate using conventional culturing methods. Exiguobacterium A1 was grown in Reasoner’s 2A (R2A) and Luria-Bertani (LB) media in both LSMMG and normal gravity (1xG) conditions to identify if there was a nutritional preference in these conditions. In addition, Exiguobacterium A1 has been sequenced, but not yet categorized. Categorization of Exiguobacterium A1 was performed with Biolog Phenotypic Microarrays, Fatty acid analysis and the transmission electron microscopy (TEM) for cellular morphology. Exiguobacterium A1 species name has been proposed as Exiguobacterium astronatus. Exiguobacterium A1 displayed higher cell concentration under 1xG in LB medium after 6 hours and between 6 to 12 hours under 1xG in R2A medium when compared to LSMMG. LSMMG plays a small role initially when growth in R2A media, but overall, Exiguobacterium A1 did not show a preference in growth under LSMMG in either medium.
Bio: Calvin was born in California and has lived in Massachusetts, Florida, and Texas. He graduated in 2016 from Texas A&M University with a Bachelor of Science in Molecular and Cell Biology. He joined Dr. McLean’s lab in the Fall of 2019 when he started his Masters’ at Texas State University. Calvin works as a registered dental assistant since 2013. Calvin will be continuing his education at Tufts University School of Dental Medicine to become a dentist. In his spare time, he enjoys attending music concerts, paddleboarding, playing video games or enjoying the outdoors with his dog, Navi.
Implications of Cropping Rate Variability on the Costs of Vigilance
Name: Elizabeth Kurpiers
Dr. Floyd Weckerly, Department of Biology, Texas State University
Committee Members: Dr. Andrea Aspbury, Department of Biology, Texas State University
Dr. Clay Green, Department of Biology, Texas State University
Thursday March 24th, 1:00 PM
Please attend in-person in W.E. (Henry) Norris, Jr. Conference Room (Supple 376) or via Zoom: https://txstate.zoom.us/j/92275934001
Abstract: Large grazing ungulates must forage for much of their active time to meet the energy demands of their massive bodies because they depend on nutrient-poor grasses and forbs. As such, maximizing efficiency of foraging bouts is selectively advantageous. Vigilance is a social and antipredator behavior that can result in reduced predation risk; however, vigilance directly competes with foraging and imposes an inherent foraging efficiency cost. As more time is allocated to vigilance, less time is available to forage. Using 199 focal observations of Roosevelt elk (Cervus elaphus roosevelti) collected in the Redwood National and State Parks of Northern California, I explored if the variability in elk cropping rate results in increased short-term forage intake and subsequently compensate for foraging time lost to vigilance. Notably, compensation via increased cropping rate is likely only feasible when food searching time is minimal and bite sizes are small, conditions that were satisfied in our study area. I developed and compared two novel models that describe possible mechanisms in which cropping rate compensation could occur. The Strategic Compensation Model illustrates a scenario where cropping rate compensation occurs due to a deliberate decision by foraging elk to increase their cropping rate. In contrast, the Incidental Cropping Variability Model describes a situation where cropping rate variability is not strategic, but rather occurs due to the influence of social and environmental factors on cropping rate. I compared the proportion of time elk spent vigilant to their cropping rate (bites . observation length-1). Four linear mixed-effect model were developed and compared using Bayesian model selection analysis. Three of the four models were identified as competing models, and each of the competing models contained the predictors: the proportion of the foraging bout spent vigilant, proportion of the foraging bout spent within one body length of a conspecific, time of day, and length of the focal observation. The influence of these predictors on cropping rate demonstrates that external factors influence cropping rate and supports the idea that cropping rate variability is incidental, likely occurs sporadically, and can compensate for vigilance in certain circumstances.
Bio: Liz was born and raised in Maple Grove, Minnesota. In 2017, She earned her B.A. in Biology and Hispanic Studies from the College of Saint Benedict, a small, liberal arts college in central Minnesota. After graduating, Liz worked at a preclinical research lab as an Associate Pharmacologist testing pain mitigation methods and drugs. Liz has a love of the outdoors, is in training to become a Master Naturalist, and has worked on field projects including sage-grouse monitoring, tree swallow surveys, and Sally-light foot crab behavioral studies. Liz joined the lab of Dr. Weckerly in 2020 and has since been studying Roosevelt elk and white-tailed deer foraging behavior.
Factors Influencing Scaling Relationships of Body and Antler Mass in White-tailed Deer (Odocoileus virginianus)
Major Advisor: Dr. Floyd Weckerly
Committee Members: Dr. Ivan Castro-Arellano, Texas State University
Dr. Clay Green, Texas State University
Friday, 25 March 2022, 3:00 PM
Attend in-person in IGRM 3104 or on Zoom https://txstate.zoom.us/j/94247385087?pwd=MHU0S1BGaWs3MjJzTXB4ZlhtNzRlZz09
Antlers are a costly trait that require skeletal reserves to grow to a large size. Thus, insight into variation in antler size requires understanding the connection between antler and body size, which can be summarized through ontogenetic and static scaling relationships. Both types of scaling relationships are needed to examine the influence of factors besides body mass on antler growth at different ages and whether the influence of these factors diminish with age. Diet variability and maternal effects might decline with age as individuals attempt to make up for size deficits through compensatory growth. I sought insight into the influences of diet and maternal attributes of dam age at birth and litter size on body-antler size relationships throughout ontogeny and at discrete ages. I also examined whether diet and maternal effects diminished with male age. Data on age, maternal characteristics, body mass, and antler mass was gathered from 168 captive, pen-raised white-tailed deer (Odocoileus virginianus) that consumed either a low energy (1.77 kcal/gm) or standard energy diet (2.65 kcal/gm) from the time they were weaned until they died by age 5.5 years of age. Both types of scaling relationships were estimated with linear mixed effects models to account for repeated measurements of males, dams, and sires. Diet affected ontogenetic scaling relationships. Males consuming the low energy diet had faster antler growth when young than old; compared to males consuming the standard energy diet. A Bayesian Information Criterion model selection analysis indicated that diet and litter size (singleton or multiple births), but not dam age at birth influenced static scaling relationships. Static scalar coefficients up to 3.5 years of age were positively allometric, but isometric in 4.5- and 5.5-year-old-males. Furthermore, diet and litter type influenced both intercepts and slopes in only the youngest males (1.5-years) lending support to the idea that diet and maternal effects diminish with age. Body size – antler size relationships are complex. Diet and litter type had greater effect at younger ages during rapid growth than at older ages. Young males that are small because of maternal effects might still be able to acquire large body and antler sizes at older ages when diets provide adequate nutrition.
Biography: Willis was born and raised in Norman, Oklahoma. He graduated from the University of Oklahoma with a B.S. in Biology in 2014. He works as a biologist for the Texas National Guard.
Announcements of past thesis and dissertation defenses can be found in the documents below. These announcements include student name, major adviser, committee members, and date, as well as an abstract of thesis or dissertation.
Announcements for calendar year: